i have an Asp web site that uses Windows authentication. It calls a web service that uses impersonation with a username and password to access the web service that resides on another server.

IIS is set to use anonymous access to the web service.

When we try to access the service through asmx interface we get

Could not load file or assembly 'classname'  or one of its dependencies. Access is denied.

the username has full control permissions. This happens only on the production side.

Any suggestions.

ASP.net version. 2.0 / IIS version 6.0

probably there is some role access issue.

If the service not lies in the root then make sure that the Directory allows the user role.