I updated the NTFS permissions for the Network Service account, the IUSR-account and the IIS_WPG account so that they all have write access to my access database.
Permissions need to be for the folder where mdb file resides, not just for the mdb file itself. (Creating the ldb, locking file needs those permissions). Did you check that?
Thanks,
Teemu Keiski
Finland, EU