No do not write it in the Master, this attribute exist only in the Page Class.
<%@ Page Language="VB" MasterPageFile="~/Template.master"AutoEventWireup="false" CodeFile="Page1.aspx.vb" Inherits="Page1" Culture="auto" UICulture="auto"%>
or you can add globalization section in the webconfig
<globalization uiCulture="auto" culture="auto" />
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Abdulla AbdelHaq MCTS, MCPD
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