Yes there is a way to validate this, just have to determine your own business rules for validation.
The button or whatever that submits the values and must validate the values must have set the attribute CausesValidation = true..
The validation rules can be describe in the Validate() method of the page. (in your case, check if a checkbox is checked).
In the OnClick event of the button you can call the Validate() method, and check if Page.IsValid == true.
Hope this will give you an idea, there are thousands of good examples on the net and msdn.
Mathijs - ASP.NET developer for Bibito project.
http://www.bibito.nl