Could not load file or assembly 'AjaxControlToolkit' or one of its dependencies. Access is denied. : The Official Microsoft ASP.NET Forums

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Chris Porter

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Re: Could not load file or assembly 'AjaxControlToolkit' or one of its dependencies. Access is de...

Jan 23, 2007 10:52 PM|LINK
Try restarting IIS and completely closing/reopening your webbrowser. I had the same error and restarting IIS corrected it.