Hi,
Did you apply the latest service pack on your machine? If not, I suggest you to apply the latest service pack.
If we insert breakpoint in your project, does it appear with a warning triangle or hollow circle and never get hit during debugging session? If so, you can get some clues from the warning message.
For more information about debugging, see http://blogs.msdn.com/jimgries/pages/visual-studio-debugger-faq.aspx (Breakpoints and other Execution Control).
Additionally, you can try to delete the your website folder under "C:\WINDOWS\Microsoft.NET\Framework\v2.0.50727\Temporary ASP.NET Files" , and then rebuild your website to see if breakpoint is hit.
I hope this helps.