Yes or No. This really depends on how the network and security is setup as well as how IIS is configured.
Are you familiar with Process Monitor?
http://www.microsoft.com/technet/sysinternals/utilities/processmonitor.mspx
It is a good tool for troubleshooting file access. If properly configured, it will tell you what process tried to access what file and whether it was successful or not.
Hope this helps.
Bruce
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